Regulatory
Part:BBa_K3032030:Design
Designed by: Denis Baronas Group: iGEM19_Vilnius-Lithuania (2019-10-14)
ColE based cUAS Gal4 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Gal4 DNA binding sequence was carefully combined with ColE promoter sequence in order not to affect promoter's activity.
Source
This promoter sequence is design by us combining ColE promoter with Gal4 cUAS DNA binding sequence (doi:10.1093/nar/gki939).